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inverted fluorescence microscope nikon eclipse te 200  (Nikon)


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    Structured Review

    Nikon inverted fluorescence microscope nikon eclipse te 200
    Inverted Fluorescence Microscope Nikon Eclipse Te 200, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inverted+fluorescent+microscope+eclipse+te/pmc11995980-63-24-23
    Average 90 stars, based on 1 article reviews
    inverted fluorescence microscope nikon eclipse te 200 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Microscopy:

    Article Title: Characterization of a new molecule capable of inhibiting several steps of the amyloid cascade in Alzheimer's disease.
    Article Snippet: Hippocampal neurons were loaded with Fluo-8 AM (1 μM in pluronic acid/ DMSO, Abcam, USA) for 30 minutes at 37oC and then washed twice with external solution (in mM: 150 NaCl, 5.4 KCl, 2.0 CaCl2, 1.0 MgCl2, 10 glucose, and 10 HEPES (pH 7.4)). .. Cells were mounted in a perfusion chamber that was placed on the stage of an inverted fluorescent microscope (Eclipse TE, Nikon) equipped with a xenon lamp and a 40X objective (22-24oC) and subsequently illuminated for 200 ms using a computer-controlled Lambda 10-2 fi lter wheel (Sutter Instruments) and regions of interest were simultaneously selected on neuronal somata containing Fluo-8 fluorescence (absorption 490 nm and emission 514 nm) in a field having usually more than 10 cells. ..

    Article Title: Gabapentin Inhibits Multiple Steps in the Amyloid Beta Toxicity Cascade.
    Article Snippet: Oligomeric β amyloid peptide (Aβ) is one of the main neurotoxic agents of Alzheimer ́s disease (AD).. Oligomers associate to neuronal membranes forming “pore-like” structures that cause intracellular calcium and neurotransmitter dyshomeostasis, leading to synaptic failure and death.. Through molecular screening targeting the C terminal region of Aβ, a region involved in the toxic properties of the peptide, we detected an FDA approved compound, gabapentin (GBP), with neuroprotective effects against Aβ toxicity.

    Article Title: Synaptotoxicity of Alzheimer beta amyloid can be explained by its membrane perforating property.
    Article Snippet: .. The cells were mounted in a perfusion chamber that was placed on the stage of an inverted fluorescent microscope (Eclipse TE, Nikon, USA). .. The cells were briefly (200 ms) illuminated using a computer-controlled Lambda 10-2 filter wheel (Sutter Instruments, USA).

    Article Title: Molecular Docking Analysis at the Human α7-nAChR and Proliferative and Evoked-Calcium Changes in SH-SY5Y Cells by Imidacloprid and Acetamiprid Insecticides.
    Article Snippet: belong to the first generation of insecticides of the neonicotinoid class (structurally similar to nicotine, i.e., NNIs).. IMI and ACE are insecticides broadly used in crop protection.. IMI is the first representative of NNIs (first-generation chloropyridyls), while ACE pertains to the second generation of chlorothiazoles.

    Article Title: Extracellular α-synuclein alters synaptic transmission in brain neurons by perforating the neuronal plasma membrane.
    Article Snippet: .. Then were placed on an inverted fluorescent microscope (Eclipse TE, Nikon, USA) and briefly illuminated (200 ms) using a computer-controlled Lambda 10-2 filter wheel (Sutter Instruments, USA). ..

    Article Title: Differential Membrane Toxicity of Amyloid-β Fragments by Pore Forming Mechanisms
    Article Snippet: A major characteristic of Alzheimer’s disease (AD) is the presence of amyloidpeptide (A ) oligomers and aggregates in the brain.. It is known that A oligomers interact with the neuronal membrane and induce perforations that cause an influx of calcium ions and enhance the release of synaptic vesicles leading to a delayed synaptic failure by vesicle depletion.. To better understand the mechanism by which A exerts its effect on the plasma membrane, we evaluated three A fragments derived from different regions of A 1-42; A 1-28 from the N-terminal region, A 25-35 from the central region, and A 17-42 from the C-terminal region.

    Article Title: Copper-uptake is critical for the down regulation of synapsin and dynamin induced by neocuproine: modulation of synaptic activity in hippocampal neurons.
    Article Snippet: Hippocampal cells were loaded with Fluo-3 AM (1 μM in pluronic acid/DMSO, Molecular Probes, Eugene, OR, USA) for 30 min at 37◦C and then washed twice with external solution, as described above, and incubated under control or experimental conditions for a maximal time of 30 min at 37◦C. .. Neurons were mounted in a perfusion chamber that was placed on the stage of an inverted fluorescent microscope (Eclipse TE, Nikon), equipped with a CCD camera (SensiCam camera, PCO, Germany) and xenon excitation lamp. .. Cells were subsequently excited for 200 ms each 2 s intervals and recorded during 5 min, using a Lambda 10-2 filter wheel (Sutter Instruments) and regions of interest (ROI) were simultaneously selected on several neuronal somata on each plate (Ex:Em; 480:510 nm) recording more than 10 cells in each experiment.

    Article Title: ?-Amyloid Causes Depletion of Synaptic Vesicles Leading to Neurotransmission Failure
    Article Snippet: Calcium Imaging Neurons were loaded with Fluo-3 AM (1 μ m in pluronic acid/dimethyl sulfoxide; Molecular Probes) for 30 min at 36 °C. .. The cells were mounted in a perfusion chamber that was placed on the stage of an inverted fluorescent microscope (Eclipse TE; Nikon) equipped with a xenon lamp and a ×40 objective (22–24 °C). .. The cells were mounted in a perfusion chamber that was placed on the stage of an inverted fluorescent microscope (Eclipse TE; Nikon) equipped with a xenon lamp and a ×40 objective (22–24 °C).

    Fluorescence:

    Article Title: Characterization of a new molecule capable of inhibiting several steps of the amyloid cascade in Alzheimer's disease.
    Article Snippet: Hippocampal neurons were loaded with Fluo-8 AM (1 μM in pluronic acid/ DMSO, Abcam, USA) for 30 minutes at 37oC and then washed twice with external solution (in mM: 150 NaCl, 5.4 KCl, 2.0 CaCl2, 1.0 MgCl2, 10 glucose, and 10 HEPES (pH 7.4)). .. Cells were mounted in a perfusion chamber that was placed on the stage of an inverted fluorescent microscope (Eclipse TE, Nikon) equipped with a xenon lamp and a 40X objective (22-24oC) and subsequently illuminated for 200 ms using a computer-controlled Lambda 10-2 fi lter wheel (Sutter Instruments) and regions of interest were simultaneously selected on neuronal somata containing Fluo-8 fluorescence (absorption 490 nm and emission 514 nm) in a field having usually more than 10 cells. ..



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